Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase
The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized
The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps
This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA
Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Sample Types
serum, blood, plasma, saliva, urine, and other related tissue liquid