Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis
Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting
Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry
Two-parameter profiles of CD vs
CFSE; and another CD vs
FSC-W
We suggest to use FSC-H vs
FSC-A
FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.